Device

Part:BBa_K658009

Designed by: Zhizhen Zhao   Group: iGEM11_XMU-China   (2011-10-01)


a bacteria population-control device with lux pR-3 driven by lacl+pL

This device is made up of three subparts: a LuxI producer, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has generated 7 bacteria population-control devices using RBSs of different strength and different lux pR mutants. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable state. Lux pR-3(BBa_K658006) was used to regulate the expression of killer protein LacZalpha-ccdB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2347


[edit]
Categories
Parameters
controlR0011
device_typesignalling
proteinsLuxI LuxR ccdB
signalling_moleculeIPTG